"Three essentials to make dreams come true: Courage to dream big, wisdom to find the right way and persistence to overcome obstacles in the journey"
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"Three essentials to make dreams come true: Courage to dream big, wisdom to find the right way and persistence to overcome obstacles in the journey"
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PATENTS
PAPERS
PUBLISHED IN PEER REVIEWED JOURNALS Levetan, CM, Upham, Lin CT, Moore PA, Kery V (2009) Automated
96-Well Purification of Hexahistidine Tagged Recombinant Proteins on MagneHis Ni2+-Particles,
Methods in Molecular Biology,
498, 129-41 (invited manuscript) Moore,
PA, Kery V (2009) High-Throughput Protein
Concentration and Buffer Exchange: Comparison of Ultrafiltration and Ammonium
Sulfate Precipitation, Methods in
Molecular Biology 498,
309-14 (invited manuscript) Sharp JL, Anderson KK, Hurst GB, Daly DS, Pelletier DA, Cannon WR, Auberry
DL, Schmoyer DD, McDonald WH, White AM, Hooker BS, Victry KD,
Buchanan MV, Kery V, Wiley HS (2008) Statistically
Inferring Protein-Protein Associations with Affinity Isolation LC-MS/MS Assays
J. Proteome Res., 6 , 3788 -3795 Lin
CT, Moore PA, Auberry DA, Landorf EV,
Peppler T, Victry KD, Collart FR, Kery
V (2006) Automated purification of recombinant proteins: Combining
high-throughput with high yield. Protein
Express. Purif., 47:16-24.
El-Shafey
A, Tolic N, Young MM, Kery V (2006)
“Zero-length” Cross-linking in Solid State as an Approach for Analysis of
Protein -Protein Interactions. Protein
Science, 15, 429-440 Frank N,
Kery V, Maclean, KN and Kraus JP (2006):
Solvent accessible cysteines in human CBS: The
role of Cysteine 431 in AdoMet binding. Biochemistry,
45, 11021-9 Markillie LM, C Lin, JN Adkins,
DL Auberry, EA Hill, BS Hooker, PA, Von
Rechenberg M, Blake BK, Ho YSJ, Zhen Y, Chepanoske
CL, Richardson BE, Nafei Xu N, Kery
V. (2005) Ampicillin/penicillin binding
protein interactions as a model drug-target system to optimize affinity
pull-down and mass spectrometric strategies for target and pathway
identification. Proteomics.
5,1764-73 Peltier JM, Askovic S, Becklin RR,
Chepanoske CL, Ho YSJ, Kery
V, Lai S, Mujtaba T, Pyne M, Robbins PB, Von
Rechenberg M, Richardson B, Savage J, Xu N, Zhen Y, Boniface JJ (2004) An integrated strategy for the discovery of drug
targets by the analysis of protein – protein interactions. Int.
J. Mass Spect.
238, 119-130 Kery
V, Savage JR, Widjaja K,
Blake BK, Conklin DR, Ho J,
Long X, von Rechenberg M, Zarembinski
TI, Boniface JJ (2003)
Expression screen by enzyme linked immunofiltration assay designed for high
throughput purification of affinity tagged proteins. Anal. Biochem., 317, 255-258. Oliveriusova
J, Kery V, Maclean KN, Kraus JP
(2002) Deletion mutagenesis of human
cystathionine b-synthase. Impact on activity,
oligomeric status, and S-adenosylmethionine regulation
J
Biol Chem. 277, 48386-94. Maclean
KN, Gaustadnes M, Oliveriusova J, Janosik M, Kraus E, Kozich V, Kery V, Skovby
F, Rudiger N, Ingerslev J, Stabler SP, Allen RH, Kraus JP (2002) High
homocysteine and thrombosis without connective tissue disorders are associated
with a novel class of cystathionine b-synthase (CBS) mutations. Hum
Mutat
19, 641-55 Janosik
M, Kery V, Gaustadnes M, Maclean K, and Kraus JP (2001) Regulation of
human cystathionine b-Synthase by S-adenosyl-L-methionine:
Evidence for two catalytically active conformations involving an autoinhibitory
domain in the C-terminal region. Biochemistry, 40, 10625-10633 Meier
M, Janosik M, Kery V, Kraus P and Burkhardt P (2001) Structure of
human cystathionine b-synthase: a unique pyridoxal
5’-phosphate-dependent heme protein. EMBO J., 20, 3910-3916 Maclean KN, Janosik M, Oliveriusova J, Kery V and
Kraus JP (2000): Transsulfuration in Saccharomyces ceerevisiae is not
dependent on heme: purification and characterization of recombinant yeast
cystathionine b-synthase. J. Inorg. Biochem. 81, 161-171 Kery
V,
Poneleit L, Meyer JD., Manning MC,
Kraus JP (1999) Binding of pyridoxal
5’-phosphate to a hemeprotein – human cystathionine b-synthase.
Biochemistry. 38, 2716-2724 Kery
V,
Poneleit L, Kraus JP (1998) Proteolytic
cleavage of human cystathionine b-synthase into evolutionary conserved
active core: Structural and functional consequences.
Arch. Biochem. Biophys. 355,
222-232 Dong
A., Kery V., Matsuidaira J, Manning MC, Carpenter J, Kraus JP (1997) Secondary
structure of human cystathionine b-synthase
in aqueous solution: Effect of ligand binding and proteolytic truncation.
Arch.
Biochem. Biophys. 344, 125-132 Kery
V,
Elleder D, Kraus JP (1995) d-Aminolevulinate increases yield and
heme saturation of human cystathionine b-synthase
expressed in Escherichia coli.
Arch. Biochem. Biophys. 316:
24-9 Stancikova
M, Kery V, Orvisky E, Cibirova L (1994) Microplate assay for hyaluronan useful in clinical
practice. Collect.
Czech. Chem. Commun. 59: 2331-41 Kery
V,
Bukovska G, Kraus JP (1994) Transsulfuration
depends on heme in addition to pyridoxal 5’-phosphate: Cystationine b-synthase
is a heme protein. J. Biol.
Chem. 269: 25283-88 Bukovska
G, Kery V, Kraus JP (1994) Expression
of human cystathionine b-synthase
in Escherichia coli: Purification and
characterization. Protein
Express. Purif. 5: 442-448 Kery
V,
Krepinsky JJ, Waren CD, Capek P, Stahl PD (1992) Ligand recognition by purified human mannose
receptor. Arch.
Biochem. Biophys. 298: 49-55 Prokopova
A, Rovensky J, Machova J, Stancikova M, Orvisky E, Kery V (1992) Effect
of vinpocetine on rat adjuvant arthritis Int.
J. Immunopath. Pharm. 5: 193-99 Orvisky
E, Kery V, Vandakova K, Stancikova M
(1992) Rapid determination of immobilized
Con A lectin activity Appl. Biochem.
Biotech.; 32 Prokopova
A., Kery V., Stancikova M., Grimova J., Capek P., Sandula J., Orvisky E.
(1993) Methyl-a-D-mannopyranoside,
mannooligosaccharides and yeast mannans inhibit development of rat adjuvant
arthritis
J. Rheumatology. 20: 673-77 Orvisky
E, Kery V, Stancikova M (1991) Specific
high-performance liquid chromatographic determination of the molecular weight
and concentration of hyaluronic acid in complex mixtures by labeled hyaluronate
binding proteins. Biomed Chromatogr.
5: 251-55 Kery
V,
Orvisky E, Stancikova M (1991) An
approach for a rapid identification and characterization of hyaluronic acid
produced by fermentation of Streptococcus
equi Carbohydr. Polymers 17: 227-30 Kery
V,
Orlovska M, Stancikova M, Risko M, Zlnay D (1992) Urinary glysosaminoglycan excretion in rheumatic diseases
Clin.
Chem. 38: 841-46 Kery
V
(1991) Lectin-carbohydrate interactions
in immunoregulation Int. J. Biochem.
23: 631-640 Kery
V,
Kogan G, Zajacova K, Slamova K, Masler L, Alfoldi J. (1991) Hydrolysis
of yeast cell wall glucan by extracellular b-1,3-glucanases from Aspergillus niger. Enzyme
Microb. Technol.
13: 87-94 Kery
V,
Kucar S, Matulova M, Haplova J, Schmidt S (1991) Immobilization of
b-D-galactosidase from Escherichia coli on cellulose beads and its use for the synthesis of
disaccharide derivatives Carbohydr.
Res. 209: 1203-9 Novotny L, Balazova E, Sandula J, Ujhazy V, Kery V (1991) Treatment of leukemia L1210 and P388 by arabinosylcytosine-polysaccharide conjugates. In. J. Cancer 47:281-4.Kery
V,
Novotny L, Tihlarik K, Haplova J, Kacurakova M, Sandula J, Balazova E. (1990) Preparation,
properties and antileukemic activity of arabinosylcytosine-polysaccharide
conjugates Int. J. Biochem. 22:
1203-9 Kery
V,
Haplova J, Tihlarik K (1990) Factors
influencing the activity and thermostability of immobilized porcine pancreatic
lipase J. Chem. Tech. Biotech.
48:
201-207 Kery
V,
Gadus P, Kucar S (1989) Enzymatic
deacetylation of peracetylated xylosides in anhydrous methanol Chem.
Papers 44: 273-79 Brtko
J, Knopp J, Kery V (1989) Study of
the role of tryptophanyl and arginyl residues in the specific binding of
3,5,3’-triodothyronine to rat liver nuclear receptors Endocrinol. Exp. 23: 259-66. Pavliak
V, Kery V, Tihlarik K, Sandula J (1989) Affinity chromatography of
anti-mannan antibodies on mannan-acrylamide
copolymers J. Chromatograph. 490:
418-25 Kery
V,
Zajacova K, Rexova-Benkova L (1988) The
effect of hydrophobic environment on horse plasma cholinesterase Biologia
43: 1095-103. Kery
V,
Zajacova K, Slamova K (1988) Affinity
chromatography of b-1,3-glucanases from Trichoderma Reesei on various affinity sorbents
Biologia 43: 691-99 Kery
V,
Bystricky S, Sevcik J, Zelinka J, (1986) Circular
dichroism of the guanyloribonuclease Sa and its complex with guanosine
3’-phosphate Biochim. Biophys. Acta
869: 75-85. Kery
V (1986)
Theoretical interpretation of the
decrease of enzyme activity during its chemical modification Coll.
Kery
V,
Both V, Zelinka J (1986) Chemical
modification of tyrosine residues in guanyloribonuclease Sa Studia
Biophysica 114: 13-22 Kery
V,
Both V, Sevcik J, Zelinka J (1986) The
number and role of histidine residues in the active site of guanyloribonuclease
Sa Gen. Physiol. Biophys. 5:
405-14 Book
Chapters: Pontow
S. E., Kery V, Stahl P. D. (1992) The
Mannose receptor. In M. Fierlander M, M. Mueckler editors. Molecular
Biology of Receptors and Transporters, Vol. 137, Part B of International
Review of Cytology. Pp221- PUBLISHED
PRESENTATIONS Both
V., Gasperik V., Kery V., Sevcik J.,
Zelinka J. Photooxidation and chemical
modification study of histidine
residues of ribonuclease Sa. Paper presented at: Proceedings of the Fifth
International Symposium on Metabolism and Enzymology of Nucleic Acids Including
Gene Manipulations, Smolenice, 1986, Eds. J. Zelinka and J. Balan, pp
109-117. RECENT MEETINGS 22nd Annual Symposium of
The Protein Society San Diego, CA July 19-23, 2008 Kery V,
Thompson A, Bonomo J, VanderMeulen D, Shockley K, Snel L: Custom
Aggregation by Cross-Linking of rhPDGF-BB Into High Molecular Weight Multimers 2008
AAPS Annual Meeting and Exposition, November 16-20, Kery
V, Thompson A, Bonomo J, VanderMuelen D, Shockley K,
Snel L: Analysis
of Custom Cross-linked High Molecular Weight Aggregates of rhPDGF-BB The 2008 SFB Fall Symposium, Liu
Y, Young CS, Nickols JC, Kery V, Goldman S, Richter
M, Marion R. Wells MR, Hart CE: Release
and Biopotency of Recombinant Human Platelet-Derived Growth Factor-BB Combined
with a Collagen Matrix for Rotator Cuff Repair IBC’s 12th Annual
Well Characterized
Biologicals Reston, VA November 10-12, 2008:Vladimir Kery, Carrie Browning,
Eric Elmquist, Kelley Nall, Ken Shockley, Ashley Thompson, David
VanderMuelen,
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