Baculovirus, Mammalian cells.
isolation and purification
by salt exclusion, affinity extraction, affinity elution, low pressure
liquid chromatography, ionic exchange, hydrophobic, chromatography,
chromatofocusing, isoelectric focusing, preparative PAGE, HPLC, FPLC,
affinity chromatography (including preparation of affinity sorbents).
analysis by biophysical methods:
UV-VIS, EPR, FTIR, CD, fluorescence, ultradialysis, analytical HPLC, light
scattering, analytical ultracentrifugation, thermal denaturation, mass
spectrometry (LC MS/MS, QTOF, FTICR MS), protein crystallization, X-ray
folding and stability.
Quantitative evaluation of protein folding, stability and protein
formulations using spectral, kinetic and other biophysical methods.
kinetics, inhibition and activation, reaction mechanisms
– protein interaction,
ligand crosslinking, ligand binding, Scatchard
– protein interactions. Tandem
affinity purification of proteins and protein complexes, immunoprecipitation,
native protein complex purification and characterization,
ultracentrifugation, light scattering, Biacore surface plasmon resonance
sequence analysis by
BLAST, BLITZ, PC-Gene, limited proteolytic cleavage, peptide isolation and
Edman sequencing, ESI MS, MALDI MS.
modification and immobilization.
Protein cross-linking, ligand-protein cross-linking, protein conjugation,
characterization of modified and immobilized proteins, use of immobilized
enzymes as biosensors or as catalysts for bioorganic synthesis.
Analytical Chemistry and Biochemistry
(protein/biomolecule) identification and quantitation (HPLC, IEC, SEC, IEF,
PAGE, MS, LC MS/MS and other spectral methods)
of complex mixtures (biological fluids, tissue homogenates)
design and development (various biophysical principles, throughputs)
analysis (signal augumentation, application of nanotechnologies)
Proteomics and process development
throughput proteomics developing
MS based automated high throughput processes of protein and protein complex
purification, identification and characterization, chemiproteomics, whole
proteome analysis, isotope tagging, high throughput LC MS, LC MS/MS, MALDI
MS analysis, microarrays
scale process development. Large
scale protein expression (200 L fermentors), purification and
characterization, process development and troubleshooting.
Design and development of
automated HT multiplexed assays and screens (ELISA, ELIFA, FRET, TR FRET,
BRET, Luminescence, nanotechnologies).
LIMS into the process
preparation and characterization of monoclonal and polyclonal antibodies
immunoprecipitation, immunoaffinity chromatography, Western blot
including development of new reagents for them
(protein, nucleic acid) modification, conjugation and immobilization
and characterization of modified and immobilized biomolecules
and large scale expression of recombinant proteins with and without
cleavable tags in bacterial expression systems
expression systems, expression optimization
complex interdisciplinary projects – combining scientific goals with
fiscal responsibility, budget tracking, project tracking, efficient project
maintaining and coordinating research collaborations
research work, managing contract projects and laboratories, GLP, GMP
skills: Goal assessment, task prioritization and assignment, conflict
resolution, mentoring scientists and technical personnel
SOPs, project design, method development, troubleshooting
and method transfer, working in GLP, GMP, FDA regulated environment