Expertise

Protein expression: Bacteria, Baculovirus, Mammalian cells.
Protein isolation and purification by salt exclusion, affinity extraction, affinity elution, low pressure liquid chromatography, ionic exchange, hydrophobic, chromatography, chromatofocusing, isoelectric focusing, preparative PAGE, HPLC, FPLC, affinity chromatography (including preparation of affinity sorbents).
Protein analysis by biophysical methods: UV-VIS, EPR, FTIR, CD, fluorescence, ultradialysis, analytical HPLC, light scattering, analytical ultracentrifugation, thermal denaturation, mass spectrometry (LC MS/MS, QTOF, FTICR MS), protein crystallization, X-ray crystallography.
Protein folding and stability. Quantitative evaluation of protein folding, stability and protein formulations using spectral, kinetic and other biophysical methods.
Enzyme kinetics, inhibition and activation, reaction mechanisms
Ligand – protein interaction, ligand crosslinking, ligand binding, Scatchard analysis., Biacore
Protein – protein interactions. Tandem affinity purification of proteins and protein complexes, immunoprecipitation, native protein complex purification and characterization, ultracentrifugation, light scattering, Biacore surface plasmon resonance
Protein sequence analysis by BLAST, BLITZ, PC-Gene, limited proteolytic cleavage, peptide isolation and Edman sequencing, ESI MS, MALDI MS.
Protein modification and immobilization. Protein cross-linking, ligand-protein cross-linking, protein conjugation, characterization of modified and immobilized proteins, use of immobilized enzymes as biosensors or as catalysts for bioorganic synthesis.

2. Analytical Chemistry and Biochemistry

Analyte (protein/biomolecule) identification and quantitation (HPLC, IEC, SEC, IEF, PAGE, MS, LC MS/MS and other spectral methods)
Analysis of complex mixtures (biological fluids, tissue homogenates)
Assay design and development (various biophysical principles, throughputs)
Trace/ultrasensitive analysis (signal augumentation, application of nanotechnologies)

3. Proteomics and process development

High throughput proteomics developing MS based automated high throughput processes of protein and protein complex purification, identification and characterization, chemiproteomics, whole proteome analysis, isotope tagging, high throughput LC MS, LC MS/MS, MALDI MS analysis, microarrays
Large scale process development. Large scale protein expression (200 L fermentors), purification and characterization, process development and troubleshooting.
Multiplex functional assays. Design and development of automated HT multiplexed assays and screens (ELISA, ELIFA, FRET, TR FRET, BRET, Luminescence, nanotechnologies).

Introducing LIMS into the process

4. Immunochemistry.

· Development, preparation and characterization of monoclonal and polyclonal antibodies

· Quantitative immunoprecipitation, immunoaffinity chromatography, Western blot

· Immunochemical assays including development of new reagents for them

5. Bioorganic Chemistry

Biomolecule (protein, nucleic acid) modification, conjugation and immobilization
Surface biochemistry
Purification and characterization of modified and immobilized biomolecules

6. Microbiology.

Microbial fermentations
Small and large scale expression of recombinant proteins with and without cleavable tags in bacterial expression systems

· High throughput expression systems, expression optimization

7. Management & Organization

Managing complex interdisciplinary projects – combining scientific goals with fiscal responsibility, budget tracking, project tracking, efficient project management style
Creating, maintaining and coordinating research collaborations
Contract research work, managing contract projects and laboratories, GLP, GMP
People skills: Goal assessment, task prioritization and assignment, conflict resolution, mentoring scientists and technical personnel
Reviewing SOPs, project design, method development, troubleshooting and method transfer, working in GLP, GMP, FDA regulated environment
Probmem Solving